德国进口狂犬病单克隆抗体（FITC）

单克隆抗狂犬病

德国进口狂犬病单克隆抗体（FITC）

Monoclonal Anti-Rabies, FITC

用直接免疫荧光技术检测狂犬病毒。单克隆抗狂犬病，FITC用于印象涂片检测（准备，例如，从可疑的动物脑组织）或用直接免疫荧光技术在细胞培养分离病毒。传统的狂犬病病毒敏感的检测以及蝙蝠相关的狂犬病病毒属，尤其是和EBLV-2 EBLV-1

Monoclonal Anti-Rabies, FITC

Detection of rabies virus using the direct immunfluores-cence technique. Monoclonal Anti-Rabies, FITC is used for detection in impression smears (prepared, for example, from brain tissues of suspicious animals) or by virus isolation in cell cultures using the direct immunofluores- cence technique. Sensitive detection of the traditional rabies virus as well as bat-associated Lyssaviruses, especially EBLV-1 and EBLV-2

德国进口狂犬病单克隆抗体（FITC）

单克隆抗体(MAb)与抗血清(又称多克隆抗体，PAb)zui主要的区别是MAb为单一种B细胞克隆所产生的一种均一的免疫球蛋白分子。所以MAb是B细胞克隆的标志，是一种*型的抗体，它的特异性是针对一个抗原决定簇的。制备单克隆抗体不能用化学分离的方法从多克隆抗体中去分离纯化得到它，而是用分离产生抗体的B细胞克隆的方法得到它。为了使B细胞克隆能在体外人工培养下长期存活并产生*均一的MAb，G．KÖhler合Milstein于1975年创立了杂交瘤方法。所以制备单克隆抗体的技术又称杂交瘤技术(hybredoma technique)。

杂交瘤技术的基本原理是用分泌抗体但不能长期培养的B细胞与能在体外长期培养并可低温保存的肿瘤细胞进行杂交。筛选得到的杂交瘤细胞应该是既能分泌抗体又有瘤细胞的特性，可长期传代培养，又可在液氮中保存的细胞。把这些细胞单克隆化，用单克隆化的杂交瘤细胞进行单克隆抗体的生产。

The most important difference between the monoclonal antibody (MAb) and the antiserum (also called the polyclonal antibody, PAb) is that MAb is a homogeneous immunoglobulin produced by a single B cell clone. Therefore, MAb is a marker of B cell clone, and is a unique type of antibody. Its specificity is directed against an antigenic determinant. The preparation of monoclonal antibodies can not be separated and purified from polyclonal antibody by chemical separation, but it is obtained by the method of cloning the B cell that separates the antibody. In order to make B cell clones capable of long-term survival in vitro and produce a fully homogeneous MAb, G.KÖ hler and Milstein established a hybridoma method in 1975. Therefore, the preparation of monoclonal antibodies is also known as hybridoma technology (hybredoma technique).

The basic principle of hybridoma technique is to hybridize B cells that secrete antibodies but can not be cultured for a long time with cancer cells that can be cultured in vitro and can be preserved at low temperature in vitro. The screened hybridoma cells should be capable of secreting antibodies and tumor cells, which can be cultured for a long time and can be stored in liquid nitrogen. These cells were mcabled and monoclonal antibodies were produced with monoclonal hybridoma cells.

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉

【】 
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

单抗制品即为单克隆抗体（Monoclonal antibody，简称McAb）制品，其由杂交瘤细胞生产，单克隆抗体其制品纯度高、专一性强、效价高，使用时可免除不同细胞及微生物种或株间血清学上的交叉反应，大大提高了诊断的特异性及敏感性。

制备过程

3.选择性培养

选择性培养的目的是筛选融合的杂交瘤细胞，一般采用HAT选择性培养基。在HAT培养基中，未融合的骨髓瘤细胞因缺乏次黄嘌呤-鸟嘌呤-磷酸核糖转移酶，不能利用补救途径合成DNA而死亡。 未融合的淋巴细胞虽具有次黄嘌呤-鸟嘌呤-磷酸核糖转移酶，但其本身不能在体外长期存活也逐渐死亡。 只有融合的杂交瘤细胞由于从脾细胞获得了次黄嘌呤-鸟嘌呤-磷酸核糖转移酶，并具有骨髓瘤细胞能无限增殖的特性，因此能在HAT培养基中存活和增殖。

4.杂交瘤阳性克隆的筛选与克隆化

在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。