抗大鼠CD45单抗

抗大鼠CD45单抗
 

 广州健仑生物科技​有限公司 

本司长期供应尼古丁（可替宁）检测试剂盒，其主要品牌包括美国NovaBios、广州健仑、广州创仑等进口产品，国产产品，试剂盒的实验方法是胶体金方法。

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抗大鼠CD45单抗

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉 

【】
【腾讯 】
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室

【企业文化宣传】

八。伏室温匀浆管中2-3深所钟。
九。每二十毫升lv仿添RNA处剂四毫升。安帽取样，用力摇十五秒。冰上伛伏十深所钟。
十。于12000°心殿深所钟离十五，火矢分为下者lv仿相、相间与水相四时。RNA全存在水相。
十一。转不过一新管水80%加无水乙醇1 /二卷（腮96-100%，室温）。大分为三十秒之当。
意：所有之心也应在室温下行。
十二。将一样装在一个五十毫升之收管HiBind®RNA马克西栏（供。。之™HiBind RNA马克西列之大量为二十五毫升（大者积可载前后。）于二千x深所钟离三木。弃过仍至法二十二。
十三。涤塔Wash Buffer吾以移液十毫升直入RNA大旋柱。在五十x g离三深所钟，弃50ml收管。此kangyi弃五十毫升收管，换一新之50ml无RNase之离管（供）免RNase污于下一步骤前。
四。以移液十毫升洗液稀释RNA II以乙醇涤塔。离心机与出流。在道中用总管十五。
8。孵化室温匀浆理中2-3分钟。
9。添加每二十毫升lv仿RNA处理剂4毫升。头盔取样，大力岌15秒钟。雪上哺育十分钟。
10。喺12000°C.离心三个字，混合物分为低酚lv仿相、相间同上水相4个阶段。RNA*保持喺水相中。
11。Transfer no more than 80% of the aqueous phase to a fresh tube and add 1/2 volume of absolute ethanol（~96-100%，room temperature）.zui大嘅速度为30秒嘅旋涡。
注意：所有嘅离心步骤应喺室温下进行。
12。将成样品组装喺一个五十毫升嘅有冇收集理HiBind®RNA马克西栏（讲吓嘢喇！）。嘅™HiBind RNA马克西部嘅zui大盖为25毫升（较大嘅体积可以载入先后。）于2000 x g离心3分钟。擗流过并继续去步骤12。
13。洗涤塔Wash Buffer我移液15毫升直接进入RNAzui大旋转柱。喺5000 x  g离心3分钟，弃50ml有冇收集理。呢个系强烈建议放弃五十毫升有冇收集理，换个新嘅50ml无RNase嘅离心理（讲吓嘢喇！）逃过RNase污染到落个步骤之前。
14。移液15毫升清洗液稀释RNA II用乙醇洗涤塔。离心机同排出流。喺步骤15中重用集合理。

Eight. 2-3 deep clocks in a room temperature homogenate.
Nine. Twenty milliliters of LV are four milliliters for adding RNA. Sample the helmet and shake for fifteen seconds. The ten volt deep ice slouch clock.
Ten. In the 12000 degree heart Temple deep Zhongli fifteen, were divided into fire bolt LV imitation phase, interphase and phase four. All RNA exists in the water phase.
Eleven. A new tube of water 80% plus 1 / two without water (96-100%, room temperature). It is divided into thirty seconds.
Meaning: all hearts should also go down at room temperature.
Twelve. The same is placed in a fifty ml collection tube HiBind RNA Maxi bar (for.. It is the HiBind RNA listed a large number of Maxi twenty-five ml (large volume can be loaded before and after.) In two thousand x deep by Miki zhongli. Discarded to law twenty-two.
Thirteen. Wash Buffer I take the washing tower pipette ten ml into the RNA spin column. Fifty x g from three deep clocks, discarded 50ml receiving tube. This protest abandons fifty milliliters to receive the tube and changes a new 50ml free RNase off tube (supply) to avoid RNase pollution before the next step.
Four. Dilute RNA II with a ten milliliter lotion for alcohol polyester pagoda.  Centrifuge and flow. The main pipe is fifteen in the road.
8. Incubate room temperature for 2-3 minutes.
9. Add 4 ml of twenty milliliter LV per milliliter for RNA treatment. The helmet sampling, Ji vigorously for 15 seconds. The snow is fed for ten minutes.
10. In 12000 ~ C. centrifugal three words, divided into low LV phenol mixture, and aqueous phase in imitation of 4 stages. RNA*保持喺水相中。
11. Transfer no more than the aqueous phase to 80% of a fresh tube and add 1/2 volume of absolute ethanol (~96-100%, room, temperature). The maximum speed of 30 seconds, vortex.
Note: all my steps should be carried out at room temperature in centrifugal.
12. The sample assembly in a fifty ml, have collected HiBind RNA Maxi column (about the scare yeah!). It's HiBind RNA Mark's biggest western 25 ml cover (larger volume, you can load it.) 2000 x g centrifugation for 3 minutes. To go through and continue to step 12.
13. Washing tower Wash Buffer I move 15 ml directly into the RNA maximum rotary column. In 5000 x g centrifuge for 3 minutes, 50ml have collected and abandoned. It is strongly recommended to give up fifty ml has been collected, a new 50ml's free RNase. From the psychological (speak ye scared!) Escape from RNase pollution until the drop step.
14. The 15 milliliter cleaning solution is diluted to RNA II with ethanol washing tower. The centrifuge is the same as the discharge flow. In step 15 set reasonable reuse.