AML1（21q22）基因断裂探针

AML1（21q22）基因断裂探针

 广州健仑生物科技​有限公司 

本司长期供应尼古丁（可替宁）检测试剂盒，其主要品牌包括美国NovaBios、广州健仑、广州创仑等进口产品，国产产品，试剂盒的实验方法是胶体金方法。

我司还有很多荧光原位杂交系列检测试剂盒以及各种FISH基因探针和染色体探针等，。

AML1（21q22）基因断裂探针

   本试剂盒主要用于AML1（21q22）基因断裂的检测，里面包括即用型杂交液和DAPI复染剂。
本试剂盒仅供科研使用。

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉 

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【腾讯 】
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室

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细胞污染是细胞培养的大敌，预防和避免污染是细胞培养成功的关键。一旦轻敌就会前功尽弃，不仅浪费时间，而且浪费人力、物力，甚至造成无法弥补的损失。而且小编的童鞋也经常向我抱怨：每到毕业季，就蓝瘦，香菇是因为细胞死了一批又一批，毕业遥遥无期啊！那么如何打一场漂亮的细胞保卫战呢？下面就跟随小编一起来学起来吧~

*步：认识细胞培养过程中有哪些污染？

细胞培养污染是指培养环境中侵入了对细胞生存有害的成分和造成细胞变异的异物。体外细胞培养污染可分为三类：物理性、化学性及生物性污染。

物理性污染：
物理性污染通过影响细胞培养体系中的生化成分，从而影响细胞的代谢。
物理性污染的来源：培养环境中的物理因素，如温度、放射线、振动、辐射（紫外线或荧光）会对细胞产生影响。
物理性污染的现象：细胞、培养液或其他培养试剂暴露在放射线、辐射或过冷过热的温度中，可以引起细胞代谢发生改变，如细胞同步化、细胞生长受抑制，甚至细胞死亡。
化学性污染
化学性污染是一些对细胞有毒性的或对细胞产生刺激的化学物质。
化学性污染的来源：
未纯化的物质、试剂、水、血清、生长辅助因子及储存试剂的容器都可能成为化学性污染的来源。细胞培养的必需养分（如氨基酸）若浓度超过了合理的范围，也会对细胞产生毒性。同样，不同细胞系在*培养条件下对血清和缓冲液的要求是不一样的，在培养中应严格控制。
化学性污染的现象：化学性污染同样会使细胞生长受抑制，甚至细胞死亡。
生物污染
生物污染包括比较容易发现的细菌、霉菌和酵母的污染，和较难发现的病毒、支原体和其他细胞的污染。
生物性污染的来源：
● 取材不慎或处理不当
● 空气尘埃
● 消毒不严
● 操作不当
● 试剂尤其是血清污染
生物性污染的现象：
● 细菌：G+菌较多。
● 真菌：霉菌较多。
● 支原体：不易检测。
● 病毒：由于病毒有种属特异性，所以病毒污染的概率比较小。

第二步：做好预防污染措施
针对细胞污染的三个分类，做好如下预防措施：
A.物理性污染的预防
● 实验室的合理设计及建立规范的操作规程；
● 培养箱应放在恒温的环境中；
● 培养液及试剂应放在固定位置，而且要注意避光，试剂周围不能放置同位素；
● 从冰箱中取出的培养液应在室温条件中放置一段时间后再进行使用，以避免过冷的温度对细胞的影响。
B.化学性污染的预防
● 使用的所有物质都应是高纯度的，同时正确配制和储存培养液及试剂。
● 在配制液体和清洗容器时必须使用不含杂质的超纯水。
● 选用同一批次的血清。
● 在选用器皿时，根据被培养细胞的生长特性、培养方式和所用培养液的性质来选用适合的器皿来达到实验的准确性和可靠性。
C.生物性污染的预防
● 建立规范的无菌操作程序及各种规章制度，并严格执行。
● 控制环境污染，同时注意实验人员的防护及无菌服的洁净和无菌。
● 保证细胞培养基和器材无菌。
● 在细胞培养基中加入适量的抗生素。
预防是防止细胞培养过程中发生污染的办法。只有提前做好预防工作，才能将发生
污染的可能性降到zui小程度。

Cell pollution is the enemy of cell culture. Preventing and avoiding pollution is the key to the success of cell culture. Once the enemy will come to naught, not only a waste of time, and a waste of manpower and material resources, and even cause irreparable loss. And little children's shoes often complain to me: every graduation season, the blue thin, letinous edodes is because of the cell dead batch after batch, graduation far away ah! So how do you fight a beautiful cell defense? Then follow the little editor to learn.

The first step: what are the pollution in the process of cell culture?

Cell culture pollution refers to the invasion of elements which are harmful to the survival of cells and the foreign bodies that cause cell mutation in the culture environment. In vitro cell culture pollution can be divided into three categories: physical, chemical and biological pollution.

Physical pollution:
Physical pollution affects the metabolism of cells by affecting the biochemical components of the cell culture system.
The sources of physical pollution: physical factors in the culture environment, such as temperature, radiation, vibration, radiation (ultraviolet or fluorescence), can affect cells.
The phenomenon of physical pollution: cells, culture fluids or other culture reagents exposed to radiation, radiation or supercooled superheated temperatures can cause changes in cellular metabolism, such as cell synchronization, cell growth inhibition, and even cell death.
Chemical pollution
Chemical pollution is a chemical substance that is toxic to cells or stimulates cells.
Sources of chemical pollution:
Containers of non purified substances, reagents, water, serum, growth cofactors and storage reagents can all be the source of chemical pollution.  The essential nutrients (such as amino acids) in cell culture, if the concentration exceeds the reasonable range, can also be toxic to the cells. In the same way, the requirements of different cell lines to the serum and buffer solution are different under the best culture conditions, and should be strictly controlled in the culture.
Chemical pollution: chemical pollution can also inhibit cell growth and even cell death.
Biological pollution
Biological pollution includes contamination of bacteria, fungi, and yeast, which are easier to detect, and the contamination of viruses, mycoplasma and other cells that are more difficult to detect.
Sources of biological pollution:
Inadvertently or mishandled
Air dust
Disinfection is not strict
Improper operation
Reagents, especially serum pollution
The phenomenon of biological pollution:
Bacteria: more G+ bacteria.
Fungi: more fungi.
Mycoplasma: it is not easy to detect.
Virus: the virus has a species specificity, so the probability of virus contamination is relatively small.

The second step: do a good job of preventing pollution
According to the three categories of cell pollution, the following preventive measures are done:
Prevention of physical pollution in A.
The rational design of the laboratory and the establishment of a standardized operating procedure.
The incubator should be placed in a constant temperature environment.
The c*tion liquid and reagents should be placed in a fixed position, and the light should be avoided, and the isotopes can not be placed around the reagent.
The culture liquid extracted from the refrigerator should be used at room temperature for a period of time to avoid the effect of the supercooling temperature on the cells.
Prevention of B. chemical pollution
All substances used should be of high purity, and the culture fluid and reagents are properly prepared and stored.
Ultra pure water containing no impurities must be used in the preparation of liquid and cleaning containers.
Select the same batch of serum.
When selecting utensils, according to the growth characteristics of the cultured cells, the way of culture and the nature of the culture medium, we choose suitable containers to achieve the accuracy and reliability of the experiment.
Prevention of C. biological pollution
The establishment of standardized aseptic procedures and various rules and regulations, and strict implementation.
Control the environmental pollution, and pay attention to the protection of the laboratory personnel and the cleanliness and asepsis of the aseptic clothes.
Ensure the asepsis of cell culture medium and equipment.
An appropriate amount of antibiotics is added to the cell culture medium.
Prevention is the best way to prevent pollution in the process of cell culture. Only if the precautionary work is done ahead of time, can it happen
The possibility of pollution is minimized.