神经9项联合检测试剂盒（PCR-荧光探针法）

神经9项联合检测试剂盒（PCR-荧光探针法）

广州健仑生物科技有限公司

Four tube multiplex for detection of cytomegalovirus, Epstein-Barr virus, adenovirus, herpes simplex virus 1, 2, varicella-zoster virus, enterovirus, parechovirus, human herpes virus 6, 7, parvovirus B19 and internal control.
四管多重检测巨细胞病毒，EB病毒，腺病毒，单纯疱疹病毒1,2型，水痘带状疱疹病毒，肠道病毒，小RNA病毒，人疱疹病毒6,7型，细小病毒B19和内部对照。

神经9项联合检测试剂盒（PCR-荧光探针法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

（1）取河沙加入10％稀盐酸，加热煮沸30分钟，以去除其中的有机质。
（2）倒去酸水，用自来水冲洗至中性。
（3）烘干，用40目筛子过筛，以去掉粗颗粒，备用。
（4）另取非耕作层的不含腐植质的瘦黄土或红土，加自来水浸泡洗涤数次，直至中性。
（5）烘干，碾碎，通过100目筛子过筛，以去除粗颗粒。
（6）按一份黄土、三份沙的比例（或根据需要而用其他比例，甚至可全部用沙或全部用土）掺合均匀，装入10×100mm的小试管或安瓿管中，每管装1g左右，塞上棉塞，进行灭菌，烘干。
（7）抽样进行无菌检查，每10支沙土管抽一支，将沙土倒入肉汤培养基中，37℃培养48小时，若仍有杂菌，则需全部重新灭菌，再作无菌试验，直至证明无菌，方可备用。
（8）选择培养成熟的（一般指孢子层生长丰满的，营养细胞用此法效果不好）优良菌种，以无菌水洗下，制成孢子悬液。
（9）于每支沙土管中加入约0．5ml（一般以刚刚使沙土润湿为宜）孢子悬液，以接种针拌匀。
（10）放入真空干燥器内，用真空泵抽干水分，抽干时间越短越好，务使在12小时内抽干。
（11）每10支抽取一支，用接种环取出少数沙粒，接种于斜面培养基上，进行培养，观察生长情况和有无杂菌生长，如出现杂菌或菌落数很少或根本不长，则说明制作的沙土管有问题，尚须进一步抽样检查。
（12）若经检查没有问题，用火焰熔封管口，放冰箱或室内干燥处保存。每半年检查一次活力和杂菌情况。
（13）需要使用菌种，复活培养时，取沙土少许移入液体培养基内，置温箱中培养。
4. Sandy soil preservation method
(1) Take sand and river add 10% dilute hydrochloric acid, heat and boil for 30 minutes to remove the organic matter therein.
(2) pour acid, rinse with tap water until neutral.
(3) drying, with 40 mesh sieve to remove coarse particles, spare.
(4) Take another non-tillage layer of humus-free thin loess or laterite, add tap water to wash several times until neutral.
(5) drying, crushing, sieved through a 100 mesh sieve to remove coarse particles.
(6) Mix well in a ratio of one loess and three sands (or in all other proportions, or even all with sand or soil, if needed) and load into small test tubes or ampoules of 10 x 100 mm each Install about 1g, stuffed tampons, sterilization, drying.
(7) Sampling for aseptic inspection, pumping one out of 10 sand soil tubes, pouring sand into broth medium, incubating at 37 ℃ for 48 hours, if there is still bacteria, you need to re-sterilize, then no Bacteria test, until proven sterile, before use.
(8) choose to c*te mature (generally refers to the spore layer full growth, vegetative cells with this method ineffective) good strains, sterile water washing, made of spore suspension.
(9) Add about 0.5ml (generally only to moistening the soil) spore suspension to each sand tube and mix it with an inoculation needle.
(10) into a vacuum desiccator, pumping the water with a vacuum pump, the pumping time is as short as possible, so that within 12 hours drained.
(11) Extract one for each 10, remove a few grit with inoculation loop, inoculate on slant culture medium, culture, observe the growth and presence or absence of bacteria growth, such as the emergence of a small number of bacteria or colonies or not at all Long, then the production of sand tube problems, still need further sampling.
(12) If there is no problem after inspection, flame-seal the nozzle, put the refrigerator or indoor dry place to save. Check the vitality and bacteria every six months.
(13) need to use bacteria, resurrection culture, take a little sand into the liquid medium, incubator incubator.
This method is mostly used to produce spores of microorganisms such as mold, actinomycetes, and therefore the most widely used antibiotic industrial production, the effect is also good, can be stored for about 2 years, but applied to vegetative cells ineffective.