诺如病毒G1/G2分型双重荧光PCR检测试剂盒

核酸试剂 诺如病毒G1/G2分型双重荧光PCR检测试剂盒

广州健仑生物科技有限公司

准备使用lyo master混合物（各8孔条），用于检测诺如病毒G1和诺如病毒G2包括内部对照。
Ready to use lyo master mix (8-well strips) for detection of norovirus G1 and norovirus G2 including an internal control

核酸试剂 诺如病毒G1/G2分型双重荧光PCR检测试剂盒

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核酸试剂

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

染色结束后，切片中见不到任何阳性信号。这是常规工作中比较常见的现象，出现这种现象，有两种可能：1、真阴性结果：整个染色过程没有出现问题，组织或细胞确实不表达与抗体相关的抗原。
2、假阴性结果：即此阴性结果不是真实的反映。假阴性结果又可分为两种情况：（1）、切片中根本就不包含所预期检查的组织或细胞。出现这种情况，要麽是病理医生选择错了切片或抗体选错了，要麽是技术员选错了蜡块。获得正确的切片进行染色是获得正确结果的前提。由此表明：制作出合格的免疫组化切片不仅仅是技术员的事，病理医生也起着*的作用。
（2）、染色过程中的某一或某些环节出了问题。比如，组织未进行抗原修复，有的组织必须经过抗原修复才能检测抗原表达；或选用了只能用于冰冻组织而不能用于石蜡包埋组织的抗体；或一抗失效，虽然抗体失效在理论上是一个逐渐的过程，但偶尔也遇到突然失效的情况，抗体长期不用和/或已超过有效期是主要的原因。也可见于染色过程中漏掉了某一环节，如忘记加二抗或三抗，或用了两次二抗而缺少了三抗，或配制DAB时少了过氧化氢。为了避免这种简单的错误，有一种简单的方法：在三抗孵育结束时，将切片上的三抗甩在一张白纸上，在将配制好的DAB滴一滴在白纸的三抗上，观察是否出现棕色。如果出现了，证明三抗和DAB的配制过程没有错误。如果这种DAB再滴到切片上没有出现任何阳性信号，问题一定是出在三抗以前。如果纸上不出现棕色反应，问题肯定在三抗DAB或DAB的配制过程。这种简单方法能迅速的帮助我们查找出现问题可能的原因。

After staining, no positive signals were seen in the sections. This is a common phenomenon in routine work. There are two possibilities for this phenomenon: 1. True negative result: There is no problem in the whole dyeing process, and the tissue or cells do not express the antibody related to the antibody.
2, false negative results: This negative result is not a true reflection. False negative results can be divided into two cases: (1), the slice does not contain the desired examination of the tissue or cells. When this happens either the pathologist chooses the wrong slice or the antibody is the wrong one, or the technician selects the wrong wax block. Getting the right slice for staining is a prerequisite for getting the right result. This shows that: to produce qualified immunohistochemical sections is not just a technician, pathologists also play an indispensable role.
(2), the dyeing process in some or some of the problems. For example, the tissue is not antigenic repair, and some organizations must undergo antigen retrieval in order to detect antigen expression; or can only be used for frozen tissue can not be used for paraffin-embedded tissue antibodies; or primary antibody failure, although the antibody failure in the theory Is a gradual process, but occasionally encountered a sudden failure, the antibody long-term useless and / or has expired is the main reason. Can also be found in the dyeing process missed a link, such as forgetting to add two or three anti-antibody, or the use of two secondary antibodies and the lack of the three anti-DAB preparation or less hydrogen peroxide. In order to avoid such a simple error, there is a simple method: the end of the third antibody incubation, the triad on the chip thrown on a piece of white paper, the preparation of a good DAB drops of white on the three anti- , Observe whether there is brown. If present, it is demonstrated that there is no error in the formulation of the third antibody and DAB. If this DAB drop to the section did not appear any positive signal, the problem must be out before the third antibody. If the paper does not appear brown reaction, the problem is certainly in the three anti-DAB or DAB preparation process. This simple method quickly helps us find out the probable cause of the problem.