诺如病毒G1/G2检测试剂盒（PCR-荧光探针法）

核酸试剂 诺如病毒G1/G2检测试剂盒(PCR-荧光探针法)

广州健仑生物科技有限公司

准备使用lyo master混合物（各8孔条），用于检测诺如病毒G1和诺如病毒G2包括内部对照。
Ready to use lyo master mix (8-well strips) for detection of norovirus G1 and norovirus G2 including an internal control

核酸试剂 诺如病毒G1/G2检测试剂盒(PCR-荧光探针法)

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核酸试剂 诺如病毒G1/G2检测试剂盒(PCR-荧光探针法)

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

方法如下： 
1．人IgG聚合物的制备 在5ml含40mg/ml人IgG的0.1mol/L pH7.0磷酸缓冲溶液中，加入2.5%戊二醛溶液1ml，边加边搅，5min即出现混浊，逐现大块胶块，放置30min后，用研钵将凝胶磨细，继用1.0mol/L pH7.0磷酸缓冲溶液反复洗涤3次，末次加蒸馏水至20ml，即为人IgG聚合物悬液。 
2．免疫吸收法
将待吸收的抗SIgG血清加入待量IgG聚合物悬液，置室温搅拌60min,离心沉淀，上清液即稀释1倍的纯化抗SIgA血清。如用IgG聚合物作少量分次吸收，其效果更好。 
（八）伊文氏蓝（Evans blue）衬染法 
用0.01%伊文氏蓝的0.01mol/L pH7.2PBS稀释荧光抗体，可将背景细胞和组织染色，呈红色荧光，与特异性黄绿色荧光形成鲜明的对比，减少了非特异性荧光，宜作常规应用。伊文氏蓝一般先配成1%溶液，保存于4℃，用前再稀释至0.01%用以和然释荧光抗体。 此外，还可以用胰酶消化组织切片或用10%牛血清蛋白封闭法等消除非特异性染色，提高特异性染色。 

Methods as below: 
1. Preparation of human IgG polymer In 5ml 0.1mol / L pH7.0 phosphate buffer solution containing 40mg / ml human IgG, add 1ml of 2.5% glutaraldehyde solution, while adding stirring, turbidity appeared 5min, After placing for 30min, the gel is ground in a mortar, washed repeatedly with 1.0mol / L phosphate buffer solution pH7.0 three times, distilled water last added to 20ml, which is human IgG polymer suspension.
2. Immunostimulation
The anti-SIgG serum to be absorbed was added to the suspension of IgG polymer to be measured, stirred for 60 minutes at room temperature, and centrifuged. The supernatant was diluted 1 time with purified anti-SIgA serum. Such as using IgG polymer as a small amount of fractional absorption, the effect is better.
(Eight) Evans blue lining method
Fluorescent antibodies were diluted with 0.01 mol / L pH 7.2 PBS at 0.01% Evans blue to stain background cells and tissues, showing red fluorescence in stark contrast to specific yellow-green fluorescence and reducing nonspecific fluorescence. Conventional application. Evans blue generally first dubbed 1% solution, stored at 4 ℃, before use and then diluted to 0.01% for natural release of fluorescent antibodies. In addition, you can try to digest tissue sections with trypsin or 10% bovine serum albumin and other non-specific staining to eliminate and improve the specific staining.