食物感染流感病毒快检卡（胶体金法）

EIKEN食物感染流感病毒快检卡（胶体金法）

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EIKEN食物感染流感病毒快检卡（胶体金法）

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【市场部】    杨永汉

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设立阳性对照是病理医生的任务或责任，而不是技术员的责任。病理医生观察了HE切片，了解切片中是否有自身对照，如果没有，就应告诉技术员采用阳性对照。因此，病理医生在免疫组化中的作用是不可忽视的。抗体未覆盖上测试组织：当多块散开的小组织染色时，可能漏掉某块组织染色。
二、“杂音”染色片
免疫组化除正常的真实的阳性信号外常常会遇到不正常的背景着色，这些非正常的着色称为“杂音”染色。“杂音”染色种类繁多，产生的原因也多种多样，为了便于说明，笔者将其归纳为下面几种。
1、全片着色
全片着色是指整个切片全都染上了颜色，着色的强度可深可浅，总之，分不清那些组织是阳性那些组织是阴性。出现这种现象的原因有：
（1）抗体浓度过高：一抗浓度过高是常见的原因之一。解决办法是，每次使用新抗体前应当对其工作浓度进行测试，使每一抗体个体化，找到适合自己实验室的理想工作浓度，既使是即用型的抗体也应如此，不能只简单的按说明书进行染色。
（2）抗体孵育时间过长或温度较高：解决办法是，严格执行操作规程，随身佩带报时表或报时钟，及时提醒，避免因遗忘而造成时间延长。现在流行的二步法（Polymer）敏感性很高，要求一抗孵育的时间不是传统的1小时，而是30分钟，因此，要根据染色结果进行调整。

Setting up a positive control is the pathologist's task or responsibility, not the technician's responsibility. The pathologist looked at the HE section to see if there was a self-control in the section, and if not, l the technician to use a positive control. Therefore, pathologists in the role of immunohistochemistry can not be ignored. Antibodies are not covered by the test tissue: When multiple small stained tissues are stained, one tissue may be left missing.
Second, "murmur" stained film
Immunohistochemistry in addition to the normal real positive signals often encounter abnormal background coloring, these non-normal coloring called "murmur" staining. There are many kinds of "murmur" stains, which are caused by many reasons. For convenience, I summarize them as follows.
1, the whole film coloring
Whole-piece coloring refers to the entire section of the color dyed, the depth of the shading can be shallow, in short, can not l those organizations are positive those organizations are negative. The reasons for this phenomenon are:
(1) antibody concentration is too high: a high concentration of anti-one is a common cause. The solution is to test the working concentration before each use of the new antibody so that each antibody is individualized to find the ideal working concentration for your laboratory, even for ready-to-use antibodies, not just simple According to the instructions for staining.
(2) antibody incubation time is too long or high temperature: The solution is to strictly enforce the rules, it is best to wear timepieces or clock, timely reminder to avoid the delay caused by the forgotten. Now the popular two-step method (Polymer) high sensitivity, requiring a first antibody incubation time is not the traditional one hour, but 30 minutes, therefore, according to the staining results to be adjusted.