流感A+B病毒诊断试剂（FUJIREBIO）
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流感A+B病毒诊断试剂（FUJIREBIO） 流感主要品牌有：日本富士（瑞必欧）、日本生研、美国BD、美国NovaBios、美国binaxNOW、英国clearview、凯必利、广州创仑等。欢迎大家，广州健仑生物科技有限公司
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流感A+B病毒诊断试剂（FUJIREBIO）
广州健仑生物科技有限公司
广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、日本积水、日本荣研、美国OSOM、芬兰、爱思普林、英国Clearview、凯必利、广州创仑等主流品牌。
流感A+B病毒诊断试剂（FUJIREBIO）
我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
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【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
（3）DAB变质和显色时间太长：DAB现用现配，如有沉渣应进行过滤后再用。配制好的DAB不应存放时间太长，因为在没有酶的情况下，过氧化氢也会游离出氧原子与DAB产生反应而降低DAB的效力，未用完的DAB存放在冰箱里几天后再用这种似乎节约的办法是不可取的。DAB的显色在显微镜下监控，达到理想的染色程度时立即终止反应。不过当染色片太多时或用染色机时，这样做似乎不现实，但至少应对一些新的或少用的抗体显色时进行监控，避免显色时间过长。
（4）组织变干：修复液溢出后未及时补充液体、染色切片太多、动作太慢、忘记滴液、滴液流失等都是造成组织变干的原因。解决的办法是操作要认真仔细，采用DAKO笔或PAP Pen在组织周围画圈，可以有效的避免液体流失，也能提高操作速度。
（5）切片在缓冲液或修复液中浸泡时间太长（大于24小时）：原因上不清楚，但现象存在。有的实验室喜欢前一天将切片脱蜡至修复，第二天加抗体进行免疫组化染色，如果将装有切片和修复液的容器放在4oC冰箱过夜，对结果无明显影响，如果放在室温，特别是炎热的夏天，会出现背景着色，因此，不可存放时间太长。
（6）一抗变质、质量差的多克隆抗体：注意抗体的有效期，过期的抗体要麽不显色要麽背景着色。用新买的抗体时设立阳性对照和用使用过的抗体作比较。
(3) DAB metamorphism and color development time is too long: DAB is best used now with sediment should be filtered before reuse. DAB prepared should not be stored for too long, because in the absence of enzymes, hydrogen peroxide will also free oxygen atoms react with DAB to reduce the effectiveness of DAB, DAB is not exhausted in the refrigerator a few days later Reusing this seemingly economical approach is not desirable. The color of DAB is best monitored under a microscope, and the reaction is stopped immediay when the desired degree of staining is achieved. However, this may not be the case when there are too many dyed sheets or when using a dyeing machine, but at least some new or less used antibodies should be monitored during their color development to avoid excessive color development.
(4) tissue dry: repair fluid overflow after the liquid is not replenished in time, too many dyed slices, the action is too slow, forget the drop, drip loss and so are the causes of tissue dryness. The solution is to be careful operation, the use of DAKO pen or PAP Pen circle around the organization, can effectively avoid the loss of liquid, but also improve operating speed.
(5) Slides immersed in buffer or repair fluid too long (more than 24 hours): the reason is unclear, but the phenomenon exists. Some labs like to deparaffinize the sections the day before to repair, and the next day add antibodies for immunohistochemical staining. If a container with sections and reconstitution fluid is placed in a 4oC refrigerator overnight, there is no significant effect on the results. If placed in Room temperature, especially hot summer, there will be background coloring, therefore, can not be stored for too long.
(6) First-class anti-aging, poor quality polyclonal antibodies: pay attention to the validity of antibodies, expired antibodies either not color or background coloring. It is best to set up a positive control with the newly purchased antibody and compare with the used antibody.
