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流感病毒快速检试剂(美国OSOM)
广州健仑生物科技有限公司
广州健仑长期供应各种流感检测试剂,包括进口和国产的品牌,主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、日本积水、日本荣研、美国OSOM、芬兰、爱思普林、英国Clearview、凯必利、广州创仑等主流品牌。
流感病毒快速检试剂(美国OSOM)
我司还提供其它进口或国产试剂盒:登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
6、DAB:
6.1 ZLI-9032/9033 DAB Kit:
使用前只需将试剂盒提供的A、B、C 三种试剂各一滴加至1ml 双蒸水中,即可获得1ml DAB 工作液,简单易用。
6.1 ZLI-9030 DAB:
6mgDAB 溶于10mlTBS(0.05M pH7.6),再加入0.1ml 浓度为3%的H2O2,过滤掉沉淀物,即可。
7、AEC:
4mg AEC 溶于1ml二甲基甲酰胺中,加入14ml 浓度为0.1M 的醋酸缓冲液(pH5.2),然后加入0.15ml 3%H2O2,过滤掉沉淀物。
8、RIPA:
1×PBS,1%NP 40,0.5 sodium deoxycholate,0.1% SDS(此液体可长期保存)。以下抑制剂以储存液方式保存,临用前加入RIPA中。
8.1 10mg/ml PMSF 异丙醇溶液(用量为10?l/ml)
8.2 Aprotinin(Sigma 产品,用量为30?l/ml)
8.3 1000mM sodium orthovanadate 冷冻液(用量为10?l/ml)
9、Blotto A:
常规使用1×PBS,5% milk,0.05% Tween 20。
10、Blotto B:
与Phosphotyrosine抗体共用,1×PBS,1% milk,0.05% Tween 20。部分实验中milk 可*去除,但可能引起背景增高。
免疫组化常见问题的处理当免疫组化染色没有出现预期结果时,应系统地查找原因,而每一次只能排除一种可能的原因。
6, DAB:
6.1 ZLI-9032/9033 DAB Kit:
Just before using the kit provided A, B, C three reagents each drop added to 1ml double distilled water, you can get 1ml DAB working solution, easy to use.
6.1 ZLI-9030 DAB:
6mgDAB dissolved in 10mlTBS (0.05M pH7.6), then add 0.1ml concentration of 3% H2O2, the precipitate can be filtered off.
7, AEC:
4 mg of AEC was dissolved in 1 ml of dimethylformamide, 14 ml of a 0.1 M acetate buffer (pH 5.2) was added, and then 0.15 ml of 3% H2O2 was added and the precipitate was filtered off.
8, RIPA:
1 × PBS, 1% NP 40, 0.5 sodium deoxycholate, 0.1% SDS (this liquid can be stored for a long time). The following inhibitors were stored as a stock solution and added to RIPA immediay before use.
8.1 10 mg / ml PMSF isopropanol solution (10? L / ml)
8.2 Aprotinin (Sigma product, 30? L / ml)
8.3 1000 mM sodium orthovanadate solution (10? L / ml)
9, Blotto A:
1 × PBS, 5% milk, 0.05% Tween 20 are routinely used.
10, Blotto B:
Shared with Phosphotyrosine antibody, 1 × PBS, 1% milk, 0.05% Tween 20. In some experiments milk can be compley removed, but may cause increased background.
Treatment of common problems with immunohistochemistry When there is no expected result of immunohistochemical staining, the reason should be systematically investigated, and only one possible reason can be excluded at a time.