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日本积水流感病毒诊断试剂盒
广州健仑生物科技有限公司
广州健仑长期供应各种流感检测试剂,包括进口和国产的品牌,主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。
日本积水流感病毒诊断试剂盒
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
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【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
(九)粘片
切下来的切片经过镜检(根据情况也可不检),将符合要求的切片粘在洁净的载玻片上。粘片时先在载玻片上滴上一小滴粘片剂(可用蛋青和甘油各50ml,加1g水杨酸混合而成),加几滴蒸馏水,然后用镊子小心把切片放在水上,在35-40℃下,用拨针将切片伸直、展平,倾斜载片,使水流去并烘干。
(十)脱蜡、染色、脱水透明和封片
粘片后要经过脱蜡、染色、再次脱水透明,然后封片*保存。下面以植物生物学中观察组织结构zui常用的番红-固绿染色法说明脱蜡、染色、脱水透明和封片的过程:
二甲苯 →二甲苯(每次均10~15min.)→1/3纯酒精+2/3二甲苯(1~2min.)→1/2纯酒精+1/2二甲苯(1~2min.)→2/3纯酒精+1/3二甲苯(1~2min.)→纯酒精两次(每次1~2min.)→95%、85%、70%、50%梯度酒精复水(每级1~2min.或更长)→用1%番红配50%酒精染色1小时→50%、70%、85%、95%梯度酒精脱水(每级1~2min.或更长)→用0.1%固绿配95%酒精复染2~5秒→95%酒精脱水1~2分钟→纯酒精两次(每次1~2min.)→2/3纯酒精+1/3二甲苯透明(1~2min.)→1/2纯酒精+1/2二甲苯透明(1~2min.)→1/3纯酒精+2/3二甲苯透明(1~2min.)→二甲苯 →二甲苯(每次均10~15min.)→加拿大树胶封片→贴标签镜检及保存。
以上是石蜡制片的一般步骤;为了获得良好的制片效果,需要制片者的大量实践并在实践中不断摸索、积累经验方可。
(Nine) sticky sheet
Cut the slice after microscopic examination (according to the situation may not be seized), will meet the requirements of the sticky piece of clean glass slides. Slice slides on the slides when slimming tablets (usable egg white and glycerol each 50ml, plus 1g salicylic acid mixture), add a few drops of distilled water, and then carefully with tweezers on the water on the slice, At 35-40 ° C, use a needle to straighten the slice, flatten, tilt the slide, and let the water flow and dry.
(10) Dewaxing, dyeing, dewatering and sealing
After the sticky sheet to go through dewaxing, dyeing, dewatering again transparent, and then keep the seal permanently. The process of observing the structure of plant tissues in plant biology, which is most commonly used for safflower-solid green staining, in dewaxing, dyeing, dewatering and sealing, is described below:
Xylene → xylene (each time 10 ~ 15min.) → 1/3 pure alcohol + 2/3 xylene (1 ~ 2min.) → 1/2 pure alcohol and 1/2 xylene (1 ~ 2min. → 2/3 pure alcohol +1/3 xylene (1 ~ 2min.) → pure alcohol twice (1 ~ 2min each time) → 95%, 85%, 70%, 50% gradient alcohol rehydration 1 ~ 2min. Or longer) → stain with 1% Safranin with 50% alcohol for 1 hour → dehydrate with 50%, 70%, 85%, 95% % Solid green with 95% alcohol counterstain 2 ~ 5 seconds → 95% alcohol dehydration 1 ~ 2 minutes → pure alcohol twice (each 1 ~ 2min.) → 2/3 pure alcohol +1/3 xylene transparent ~ 2min.) → 1/2 pure alcohol +1/2 xylene clear (1 ~ 2min.) → 1/3 pure alcohol + 2/3 xylene clear (1 ~ 2min.) → xylene → xylene Average 10 ~ 15min.) → Canadian gland film → labeling and preservation.
The above is the general steps of paraffin production; in order to obtain a good production effect, the producer needs a lot of practice and in practice continue to explore and gain experience before formulating.