流感A病毒抗原快速检测试剂盒

流感A病毒抗原快速检测试剂盒

广州健仑生物科技有限公司

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流感A病毒抗原快速检测试剂盒

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

杀死与固定
利用药剂迅速把细胞杀死，以保持材料本来的状态和结构。
常用的固定有F.A.A固定液、卡诺固定液等。将固定液放在具塞小瓶中，投入材料，盖好瓶盖。用F.A.A固定液固定，时间至少24小时。F.A.A固定液既是良好的固定剂，也是保存剂，因此材料可长期保存在固定液中备用。卡诺固定液穿透力强，固定较小材料一般1~6小时即可，zui多不超过24小时。因卡诺固定液不能做保存剂，所以固定后要用95%、85%酒精浸洗，每级约20min.，然后保存在70%的酒精中备用。
（三）脱水
脱水的目的是除去组织中的水分，便于透明、浸蜡、包埋等步骤的进行，因为这些程序中所用的药品都不能与水混合，所以必须脱水。常用的脱水剂为酒精。脱水过程应缓慢进行，不能操之过急或时间过长，以免组织变硬变脆或发生收缩现象。脱水采用等级脱水（系列脱水），即从较低浓度酒精开始，逐渐替换到高浓度酒精。一般为：50%→70%→80%→95%（此时可加少许番红，使组织着色便于包埋时定位）→*（两次），每级停留时间2~4小时或更长（*中时间宜短）。
用95%酒精配制各浓度酒精的方法：设原有酒精浓度为A，所需配制酒精浓度为B，则取Bml原有酒精，加上A-Bml蒸馏水，即可获得Aml的B%的究竟。例如：95%酒精配成70%的酒精，则取70ml的95%酒精，加上25ml蒸馏水，则配成了95ml的70%的酒精。

Kill and fix
Kill the cells quickly with the agent to maintain the original state and structure of the material.
Commonly used fixed F.A.A fixative, Carnot's fixative and so on. Put the fixative solution in a vial, insert the material, and close the cap. Fix with F.A.A fixative for a period of at least 24 hours. F.A.A fixative is both a good fixative, but also preservatives, so the material can be stored in the stationary fluid long-term backup. Carnot fixing fluid penetration, fixed smaller materials generally 1 to 6 hours, up to no more than 24 hours. Incananoer fluid can not be preservatives, so fixed to 95%, 85% alcohol dipped, each level of about 20min., And then stored in 70% alcohol reserve.
(Three) dehydration
The purpose of dehydration is to remove moisture from the tissue and to facilitate the steps of clearing, dipping and embedding. Since the drugs used in these procedures can not be mixed with water, they must be dehydrated. Common dehydrating agent for alcohol. Dehydration process should be carried out slowly, can not be too hasty or too long, so as not to harden the tissue becomes brittle or shrink phenomenon. Dehydration using the level of dehydration (dehydration series), that is, starting from the lower concentration of alcohol, gradually replaced by high concentrations of alcohol. General: 50% → 70% → 80% → 95% (at this time can add a little saffron, so that when the tissue is easy to embed localization positioning) → anhydrous alcohol (twice), each level of stay 2 to 4 hours or Longer (anhydrous alcohol in time should be short).
95% alcohol preparation of the concentration of alcohol method: Let the original concentration of alcohol A, the required concentration of alcohol prepared B, Bml then take the original alcohol, add A-Bml distilled water, you can get Bml Aml . For example: 95% alcohol with 70% alcohol, then take 70ml of 95% alcohol, add 25ml of distilled water, then dubbed 95ml of 70% alcohol.