- 产品描述
人类体外检测麻疹病毒诊断试剂盒
英文名称:American FUCUS measles virus diagnostic kit
广州健仑生物科技有限公司
(广州健仑生物科技有限公司是集研制开发、销售、服务于一体的优良企业,公司产品涉及临床快速诊断试剂、食品安全检测试剂,违禁品快速检测,动物疾病防疫检测试剂,免疫诊断试剂、临床血液学和体液学检验试剂、微生物检验试剂、分子生物学检验试剂、临床生化试剂、有机试剂等众多领域,同时核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名诊断产品集团公司产品,致力于为商检单位、疾病预防控制中心、海关出入境检疫局、卫生防疫单位,缉毒系统,戒毒中心,检验检疫单位、生化企业、科研院所、医疗机构等机构与行业提供*、高品质的产品服务。此外,本公司还开展食品、卫生、环境、药品等多方面的第三方检测服务。)
主要用途:用于定量测定人血清、脑脊液或血浆中的麻疹抗体。
产品规格:96T/盒
存储条件:4-8℃
保质期:18个月
【酶联免疫法的原理】
①使抗原或抗体结合到某种固相载体表面,并保持其免疫活性。
②使抗原或抗体与某种酶连接成酶标抗原或抗体,这种酶标抗原或抗体既保留其免疫活性,又保留酶的活性。在测定时,把受检标本(测定其中的抗体或抗原)和酶标抗原或抗体按不同的步骤与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与其他物质分开,*后结合在固相载体上的酶量与标本中受检物质的量成一定的比例。加入酶反应的底物后,底物被酶催化变为有色产物,产物的量与标本中受检物质的量直接相关,故可根据颜色反应的深浅来进行定性或定量分析。由于酶的催化频率很高,故可极大地放大反应效果,从而使测定方法达到很高的敏感度。ELISA可用于测定抗原,也可用于测定抗体。
① antigen or antibody to a solid surface of the carrier, and maintain its immunocompetence.
② the antigen or antibody connected with an enzyme labeled antigen or antibody, this enzyme-labeled antigen or antibody retains both its immunological activity, but also retains the enzyme activity. In the measurement, the test sample (measured in the antibody or antigen) and the enzyme-labeled antigen or antibody in different steps with the solid phase carrier antigen or antibody reaction. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by the washing method. * The amount of enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the specimen. After adding enzyme reaction substrate, the substrate is enzymatically converted into a colored product. The amount of the product is directly related to the amount of the tested substance in the sample. Therefore, qualitative or quantitative analysis can be conducted according to the color reaction depth. Due to the high catalytic frequency of the enzyme, it is possible to greatly amplify the reaction effect so that the determination method achieves a high sensitivity. ELISA can be used to determine the antigen can also be used to determine the antibody.
【检测原理】
The immunological processes involved in ELISA (enzyme-linked immunosorbent assay) are especially confirmed in the area of infection testing for antibodies. This detection reaction is based on the specific interaction of antibodies and antigens. To this end, binding antibodies coated in the patient's sample are present in test strips of microtitre plates of Cyurotype ELISA classic. Other alkaline phosphatase secondary antibody was used to detect the resulting immune complexes. The enzyme catalyzes a reaction, the conversion of p-nitrophenol in the colored product of the colorless substrate p-nitrophenylphosphate. The signal intensity of the reaction product is detected photometrically proportional to the concentration of antibody in the sample.
人类体外检测麻疹病毒诊断试剂盒
【试剂盒的组成】
试剂盒组成 | IgG试剂盒 IgM试剂盒 IgA试剂盒 数量 / 容积 |
微孔条(此微孔条可拆下单独使用,每条有8孔,共96孔,已经包被了抗原) 1个微孔条框架 包被材料未被激活 | 12 12 12 |
标准血清(立即可用) 人血清溶于含蛋白的磷酸盐缓冲液;抗HIV抗体、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗体均为阴性; 防腐剂:< 0.1% * 染色剂:紫红色O | 2×2毫升 2×2毫升 2×2毫升 |
阴性对照血清(立即可用) 人血清溶于含蛋白的磷酸盐缓冲液;抗HIV抗体、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗体均为阴性; 防腐剂:< 0.1% * 染色剂:里沙明绿 V | 1×2毫升 1×2毫升 1×2毫升 |
酶标记的抗人IgG, IgA, IgM (立即可用) 羊抗人IgG, IgA, IgM(多克隆),标记碱性磷酸酶后在蛋白稳定剂中储存 防腐剂: 0.01% 甲基异噻唑啉酮 0.01% 溴化硝基二垩烷 | 13毫升 13毫升 13毫升 |
浓缩洗液(可稀释至1000毫升) 氯化钠溶液,含吐温20和30mM Tris 防腐剂: < 0.1%* | 1×33.3毫升 1×33.3毫升 1×33.3毫升 |
稀释缓冲液 磷酸盐缓冲液,内含蛋白和吐温20 防腐剂: < 0.1%* 0.01克 /升的溴酚蓝钠盐 | 2×50毫升 2×50毫升 2×50毫升 |
终止液 1.2N 氢氧化钠 | 15毫升 15毫升 15毫升 |
底物(立即可用) 对硝基苯磷酸盐,不含其它溶剂的缓冲液 防腐剂:< 0.1% * (未开封瓶子中的底物可能会轻微变黄,但不会影响其质量) | 13毫升 13毫升 13毫升 |
带有标准曲线和评估表的质量控制文件 (抗体以IU/毫升或U/毫升计量) | 1 1 1 |
我司同时还提供、美国FOCUS、西班牙DIA、美国trinity等试剂盒:
麻疹、风疹、甲流 、乙流、单疱疹1型、单疱疹2型、百日咳、百日咳毒素、腮腺炎、带状疱疹、单纯疱疹、HSV1型特异性、巨细胞-特异、风疹-特异、弓形虫-特异、棘球属、嗜肺军团菌、破伤风、蜱传脑炎、幽门螺旋杆菌、白色念珠菌、博氏疏螺旋体、细小病毒、钩端螺旋体、腺病毒、Q热柯克斯体、烟曲霉菌、埃可病毒、EB病毒、衣原体、耶尔森菌、空肠弯曲杆菌、炭疽杆菌、白喉、肠道病毒、柯萨奇病毒、肺炎衣原体、沙眼衣原体、土拉弗朗西斯菌、汉坦病毒、类风湿因子、呼吸道合胞病毒、单纯疱疹病毒质控品、巨细胞质控品、弓形虫质控品、风疹麻疹质控品等试剂盒以。
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【公司名称】 广州健仑生物科技有限公司
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Capsules Many bacterial cell walls surround a thick layer of thick, jelly-like material. The capsule of most bacteria (such as pneumococcal, meningococcal, etc.) consists of polysaccharides. In addition to the capsule to help identify bacteria, but also protect the bacteria from phagocytic phagocytosis and digestion, and thus the toxicity of bacteria. The mechanism of anti-phagocytic capsule is not very clear, probably due to the capsule mucus layer is relatively smooth, easy to capture by phagocytes. The capsule can store moisture to make the bacteria resistant to drying, and have some resistance to the infringement of other factors (such as lysozyme, complement, antibodies, antibacterials, etc.).
2. Flagella (Flagllum) In some bacterial cells with elongated and curved filaments, called flagella. Flagella are bacterial organs of movement, tend to have chemical tendencies, often toward a high concentration of nutrients in the direction of moving away from its harmful environment.
3. Pilus Pilus is a filamentous appendage that is thinner, shorter, straighter, harder, and more numerous than flagella over the surface of many Gram-negative bacteria, also known as Fimbriae. Pili has nothing to do with exercise. Pili can be divided into common pili (Commonpilus) and sexual pili (Sexpilus) two.
4. Spore (Bacillus) under certain conditions, Bacillus (such as Bacillus anthracis) and Clostridium (such as tetanus, gas gangrene pathogen) in the body to form a highly refractories difficult to color small Body, called the endospore (Endospore), referred to as spores.
5. Bacteria can be reproductive asexual or genetic recombination in two ways, the bacteria are generally a simple two-division method of asexual reproduction, individual bacteria, such as Mycobacterium tuberculosis occasionally branch propagation. Aseptic propagation of the two fissions, the salient features of its breeding fast. The generation of E. coli (the necessary time for bacterial doubling doubling) is 20 minutes, calculated in accordance with this, under the best conditions after 8 hours, a cell can proliferate to 2 million, after 10 hours can exceed 1 billion, 24 hours After that, the number of bacterial multiplication can be huge enough to count the data and extent.
6. Individual cells also undergo genetic variation in several ways: mutations (where the genetic code of the cell itself changes randomly), transformation (transfer of bacterial DNA from one bacterium to another bacterium in solution), transfection (virus Or bacterial DNA, or DNA from both, by phage transfer into another bacterium), bacterial conjugation (the DNA of one bacterium binds to another bacterium by the special protein structure formed between the two bacteria, which binds the pili) . Bacteria can get DNA in these ways, then divide and pass on the recombinant genome to offspring. Many bacteria contain plasmids that contain extrachromosomal DNA.