西班牙DIA喉炎麻疹IgM ELISA检测试纸

西班牙DIA喉炎麻疹IgM ELISA检测试纸

英文名称：American FUCUS measles virus diagnostic kit

广州健仑生物科技有限公司

（广州健仑生物科技有限公司是集研制开发、销售、服务于一体的优良企业,公司产品涉及临床快速诊断试剂、食品安全检测试剂，违禁品快速检测，动物疾病防疫检测试剂，免疫诊断试剂、临床血液学和体液学检验试剂、微生物检验试剂、分子生物学检验试剂、临床生化试剂、有机试剂等众多领域，同时核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名诊断产品集团公司产品，致力于为商检单位、疾病预防控制中心、海关出入境检疫局、卫生防疫单位,缉毒系统，戒毒中心，检验检疫单位、生化企业、科研院所、医疗机构等机构与行业提供*、高品质的产品服务。此外，本公司还开展食品、卫生、环境、药品等多方面的第三方检测服务。）

主要用途：用于定量测定人血清、脑脊液或血浆中的麻疹抗体。

产品规格：96T/盒

存储条件：4-8℃

保质期：18个月

【麻疹的介绍】

麻疹喉炎时的喉梗阻属于急性喉梗阻，病情发展快，苦处理不当或不及时，死亡率很高，因此患儿家长或保育人员应了解急性喉梗阻的表现，以便及时送患儿就诊。医生和护士应掌握喉梗阻分度，会判断轻重，以便不失时机地给予正确治疗。急性喉梗阻临床上分4度。I度较轻，Ⅳ度极重。
I度：患儿安静时无症状，活动或哭闹时则出现轻度吸气性呼吸困难及三凹征(胸骨上窝、锁骨上窝及肋间隙于吸气时凹陷)，心率与心音无大改变。
Ⅱ度：安静时即有轻度吸气性呼吸困难，稍活动即加重。心率稍快但心音性质正常。对睡眠和进食影响不大。缺氧症状不明显。
Ⅲ度：安静时呼吸困难及三凹征即较明显，甚至伴喘鸣音，同时出现缺氧症状如烦躁、拒食、难以入睡。心率明显增快,心音稍钝，口唇及指(趾)端呈轻微紫绀。
Iv度：呼吸极度困难，紫绀明显。同时出现意识障碍，患儿异常烦躁，手足乱动，可发展为半昏迷以至昏迷。心率可增快，也可减慢或出现心律不齐，心音低钝。 

Throat obstruction of laryngitis belongs to acute laryngeal obstruction, the rapid progression of the disease, improper or improper treatment or not timely, the mortality rate is high, so children with parental or childcare staff should understand the performance of acute laryngeal obstruction in order to promptly send children to treatment. Doctors and nurses should master the classification of laryngeal obstruction, will judge the severity, so as to seize the opportunity to give the correct treatment. Acute laryngeal obstruction clinically 4 degrees. I degree is light, Ⅳ degree extremely.
I degrees: children with asymptomatic quiet, active or crying when there is mild inspiratory difficulty breathing and three concave signs (suprasternal fossa, supraclavicular fossa and intercostal space in the suction depression), heart rate and heart sound Big change.
Ⅱ degree: quiet when there is mild inspiratory difficulty breathing, a slight increase in activity. Heart rate slightly faster but the nature of heart sounds normal. Little effect on sleep and eating. Hypoxic symptoms are not obvious.
Ⅲ degree: quiet breathing difficulties and the three concave sign is more obvious, or even with wheezing, hypoxia symptoms such as irritability, antifeedant, difficult to fall asleep. Heart rate was significantly faster heart sound blunt, lip and finger (toe) end was slightly cyanotic.
Iv degrees: extremely difficult to breathe, cyanosis obvious. At the same time there is disturbance of consciousness, children with abnormal irritability, hand-foot movement, can develop into a semi-coma and even coma. Heart rate can be increased, can also slow down or arrhythmia, low heart sound blunt.

【注意事项】　　

Only the complete use of reagents to ensure that the test results, because all reagents are related, can not be mixed with other manufacturers of products. Especially standard serum, control serum and enzyme markers, must be equipped with the kit, do not use other batch products. Dilution buffer, wash solution, stop solution, and substrate solution are used for all Serun ELISA classic kits.
 
All reagents in the ELISA classic kit must be properly stored. It should be stored at 2-8 ° C in the unopened condition and used for a period of validity (see label instructions). Detailed stability and storage information will be described in detail in "8. Storage and Stability".
 
Each reagent is calibrated to ensure optimal test results. The lack of regulation or dilution of these reagents can lead to a decrease in the sensitivity of the assay.
 
Avoid exposing reagents to bright light during storage and incubation. All reagent bottle caps should be tightly closed to prevent evaporation and contamination and reagents should be free from microbial contamination as proteolytic enzyme interference will result in erroneous results.
 
Please cut the sealed bag from the mark. If the aluminum bag is damaged or the aluminum bag with the desiccant is opened but not closed by the clip, do not continue to use the microwell.
 
Allow all reagents to room temperature before starting the test.
 
When taking reagents from the reagent tube, care should be taken to use aseptic technique to prevent contamination. To avoid false-positive results, aspiration of the enzyme conjugate, the nozzle should be kept out of contact with the eyelet or sprayed on it, taking care not to mistake the cap or cap.
 
The repeatability of the test results depends on the well-mixed reagents. Shake the vial containing control serum and all diluted solution (eg using a mixer) before use.
 
Carefully pipet the reagents and strictly observe the given incubation time and temperature. Note that when the specimen / control serum, enzyme conjugate, or substrate is aspirated, the time interval between the first well and the last well sample will result in a different "pre-incubation" time, Affect the accuracy and repeatability of the measured values.
 
If the test does not strictly abide by the validity of the control quality control certification documents specific guidelines, the test results invalid.
 
Inadequate washing will affect the test results:
 
Care should be taken to wash. The washing procedure should be carried out in accordance with the instruction manual for the relevant scrubber (flat bottom hole, hole diameter 7 mm, depth 10.9 mm). It is important that all wells be filled with the same volume of wash buffer. At the end of the wash, the microplate should be back-loaded onto a paper towel and tapped gently to ensure that no wash buffer is present in all wells. Avoid bubbles! If using an automatic plate washer, pay attention to correct operation.

【检测原理】

ELISA（酶联免疫吸附测定）是涉及的免疫学过程在抗体检测的感染领域尤其得到证实。该基于抗体和抗原的特异性相互作用的检测反应。至为此目的，使用赛润ELISA classic的微量滴定板的测试条传染性病原体特异性抗原在患者样品中的结合包被的抗体存在。 其他用碱性磷酸酶标记二抗检测由此形成的免疫复合物。 该酶催化a反应过程中，无色底物对硝基苯磷酸酯在有色产物中对硝基苯酚转化。 反应产物的信号强度正比于样品中的抗体浓度用光度法检测。

西班牙DIA喉炎麻疹IgM ELISA检测试纸

【试剂盒的组成】

我司同时还提供、美国FOCUS、西班牙DIA、美国trinity等试剂盒：

麻疹、风疹、甲流 、乙流、单疱疹1型、单疱疹2型、百日咳、百日咳毒素、腮腺炎、带状疱疹、单纯疱疹、HSV1型特异性、巨细胞-特异、风疹-特异、弓形虫-特异、棘球属、嗜肺军团菌、破伤风、蜱传脑炎、幽门螺旋杆菌、白色念珠菌、博氏疏螺旋体、细小病毒、钩端螺旋体、腺病毒、Q热柯克斯体、烟曲霉菌、埃可病毒、EB病毒、衣原体、耶尔森菌、空肠弯曲杆菌、炭疽杆菌、白喉、肠道病毒、柯萨奇病毒、肺炎衣原体、沙眼衣原体、土拉弗朗西斯菌、汉坦病毒、类风湿因子、呼吸道合胞病毒、单纯疱疹病毒质控品、巨细胞质控品、弓形虫质控品、风疹麻疹质控品等试剂盒以。

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    荚膜（Capsule）许多细菌胞壁外围绕一层较厚的粘性、胶冻样物质。大多数细菌（如肺炎球菌、脑膜炎球菌等）的荚膜由多糖组成。 荚膜除对鉴别细菌有帮助外，还能保护细菌免遭吞噬细胞的吞噬和消化作用，因而与细菌的毒力有关。荚膜抗吞噬的机理还不十分清楚，可能由于荚膜粘液层比较光滑，不易被吞噬细胞捕捉之故。荚膜能贮留水分使细菌能抗干燥，并对其他因子（如溶菌酶、补体、抗体、抗菌药物等）的侵害有一定抵抗力。
2.    鞭毛（Flagllum）在某些细菌菌体上具有细长而弯曲的丝状物，称为鞭毛。鞭毛是细菌的运动器官，往往有化学趋向性，常朝向有高浓度营养物质的方向移动，而避开对其在害的环境。
3.    菌毛（Pilus）菌毛是许多革兰氏阴性菌菌体表面遍布的比鞭毛更为细、短、直、硬、多的丝状蛋白附属器，也叫做纤毛（Fimbriae)。菌毛与运动无关。菌毛可分为普通菌毛(Commonpilus)和性菌毛(Sexpilus)两种。
4.    芽胞（Spore）在一定条件下，芽胞杆菌属（如炭疽杆菌）及梭状芽胞杆菌属（如破伤风杆菌、气性坏疽病原菌）能在菌体内形成一个折光性很强的不易着色小体，称为内芽胞（Endospore），简称芽胞。
5.    细菌可以以无性或者遗传重组两种方式繁殖，细菌一般以简单的二分裂法进行无性繁殖，个别细菌如结核杆菌偶有分枝繁殖的方式。二分裂方式无性繁殖，其突出的特点为繁殖速度极快。大肠杆菌的代时(细菌分裂倍增的必须时间)为20分钟，以此计算，在*条件下8小时后，1个细胞可繁殖到200万上，10小时后可超过10亿，24小时后，细菌繁殖的数量可庞大到难以计数据和程度。